I have been using one for a year or two and its still gtg. I mostly got mine after reading through "yeast" it is of the author's expertise that o2 saturation is huge at the beginning of the fermentation.
by levader 2021-12-10
Always do a streak plate first to get isolated colonies. Then aseptically transfer 8-10 of the most uniform colonies to 5 mL sterile media, then 50 mL, then 500 mL allowing for 24 hrs of growth in each volume. The exact volume isn't super critical, but increasing each by a factor of 10 is typical.
Highly recommend the Yeast book from Brewing Elements series:
https://www.amazon.com/Yeast-Practical-Fermentation-Brewing-Elements/dp/0937381969
by JamesAGreen 2019-11-17
There is plenty of great material in this book that is relevant to meadmakers and I highly recommend it: https://www.amazon.com/Yeast-Practical-Fermentation-Brewing-Elements/dp/0937381969 Chris' bonafides are not in question and the book is geared towards home producers. I have my MSc in Molecular Biology and I've used this book as a reference text, but YMMV.
here you go
I have been using one for a year or two and its still gtg. I mostly got mine after reading through "yeast" it is of the author's expertise that o2 saturation is huge at the beginning of the fermentation.
Always do a streak plate first to get isolated colonies. Then aseptically transfer 8-10 of the most uniform colonies to 5 mL sterile media, then 50 mL, then 500 mL allowing for 24 hrs of growth in each volume. The exact volume isn't super critical, but increasing each by a factor of 10 is typical.
Highly recommend the Yeast book from Brewing Elements series: https://www.amazon.com/Yeast-Practical-Fermentation-Brewing-Elements/dp/0937381969
There is plenty of great material in this book that is relevant to meadmakers and I highly recommend it: https://www.amazon.com/Yeast-Practical-Fermentation-Brewing-Elements/dp/0937381969 Chris' bonafides are not in question and the book is geared towards home producers. I have my MSc in Molecular Biology and I've used this book as a reference text, but YMMV.
https://www.amazon.com/Yeast-Practical-Fermentation-Brewing-Elements/dp/0937381969/ref=nodl_